Journal: iScience

Article Title: Diversity and evolution of tyrosinase enzymes involved in the adhesive systems of mussels and tubeworms

doi: 10.1016/j.isci.2024.111443

Figure Lengend Snippet:

Article Snippet: ExPASY Translate online tool , SIB (Swiss Institute of Bioinformatics) , https://web.expasy.org/translate/.

Techniques: Recombinant, Labeling, Sample Prep, DC Protein Assay, Reverse Transcription, Sequencing, In Situ Hybridization, Software

Journal: Scientific Reports

Article Title: Impaired myogenesis in limb girdle muscular dystrophy type 2B

doi: 10.1038/s41598-025-10205-9

Figure Lengend Snippet: Silencing of the DYSF gene by CRISPR/Cas9 successfully reduces the synthesis of the dysferlin protein in myotubes from the control cell line. ( A ) Immunofluorescence staining indicating the absence of DYSF in the edited cell line, and the presence of myosin, showing that the cells maintain their capacity to differentiate. ( B ) Protein detection by western blotting highlights the reduction of the dysferlin protein to the levels of the LGMD2B myotubes and shows a secondary reduction of the calpain-3 protein, a finding already observed in other LGMD2B patients. Internal WB control for protein content in each sample can be observed with labelling to dystrophin (DMD), dysferlin (DYSF) and calpain3 (CAPN3).

Article Snippet: Sanger sequencing was performed to identify possible mutations and estimate the efficiency of gene editing, and we analyzed the .abi resulting files in the online software ICE CRISPR Analysis Tool (Synthego, www.synthego.com/products/bioinformatics/crispr-analysis ).

Techniques: CRISPR, Control, Immunofluorescence, Staining, Western Blot